Season One
| Rino Romano ............. Peter Parker / Spider-Man |
| Kathleen Barr ................... Additional Voices |
| Jim Byrnes ...................... Additional Voices |
| Gary Chalk ........................... Mr. Meugniot |
| Paul Dobson ............................ The Hunter |
| Brian Drummond ................ Venom / Eddie Brock |
| Michael Donovan ........... Carnage / Cletus Kasady |
| Christopher Gaze ................... Daniel Bromley |
| Ron Halder ................................ Sir Ram |
| Jennifer Hale ...... Mary Jane Watson / Lady Vermin |
| Kim Hawthorne ...................... Karen O'Malley |
| Rhys Huber ..................... Shane Yamada-Jones |
| Janyse Jaud ..................... Additional Voices |
| Blu Mankuma ..................... Additional Voices |
| Scott McNeil .......................... The Vulture |
| Akiko Ann Morison .......... Dr. Naoko Yamada-Jones |
| Richard Newman ... J.J. Jameson / High Evolutionary |
| John Payne ................... Colonel John Jameson |
| Tasha Simms ............................. Lady Ursa |
| David Sobolov .......................... Lord Tyger |
| Alec Willows .................... Additional Voices |
| Dale Wilson .................... X-51 / Machine Men |
